normal human foreskin fibroblasts (bj)  (European Collection of Authenticated Cell Cultures)

Journal: Scientific Reports

Article Title: Chitosan-dextran sulfate nanocapsules for enhanced tigecycline efficacy against non-typhoidal Salmonella enterica

doi: 10.1038/s41598-026-35229-7

Figure Lengend Snippet: TGC and/or unloaded CD NPs ameliorated the S. Typhimurium infected group induced histopathological alterations in mice’s liver tissues. Representative photomicrographs of the H&E-stained hepatic tissue sections showing the control (A), S. Typhimurium infected group (C), TGC (E), unloaded CD NPs (G), CD-TGC groups (I) and their respective higher magnifications (B, D, F, H, and J). A, B: control group displaying normal central vein (CV), hepatic cords (HC) with hepatocytes of eosinophilic granular cytoplasm (EC), rounded central single (SN) or double vesicular nuclei (DN), and kupffer cells (KC). C, D: S. Typhimurium infected group demonstrating multiple areas of variable-sized necrotic areas (NA) of coagulative necrosis (CN), severely dilated and congested sinusoid (SDS) with Kupffer cell hyperplasia (KCH). E, F: TGC group displaying moderately sized necrotic areas (MNA), moderately dilated and congested sinusoids (MDS), moderately hyperplastic Kupffer’s cells (MKC), interstitial mononuclear cell infiltration (MI), fibroblast proliferation (FP), and regenerated hepatocytes of stippling basophilic cytoplasm (BC) and pale nuclei (PN). G, H: unloaded CD NPs group showing a few scattered minute necrotic areas (mNA), intense mononuclear cell infiltration (IMI) around the portal area, and apparently normal hepatocytes (NH). I, J: CD-TGC group showed normal hepatocytes (NH), few dilated blood vessels (DBV), and a few interstitial lymphocytic aggregates (FL). Scale bars = 100 μm in A, C, E, G, I; and = 20 μm in B, D, F, H, and J. K: Bar charts demonstrate the statistical analysis of the comparative quantification of the hepatic injury scores in all studied groups. Bars carrying different superscript letters (a, b, c, d, and e) are significantly different as analyzed by the one-way ANOVA test, followed by the multiple comparisons by Duncan’s Post-hoc test ( p < 0.05). Values are the mean of 6 mice per group ± S.E.M.

Article Snippet: BJ normal human foreskin primary fibroblast cell line (ATCC CRL-2522) was used for studying safety of CD-TGC nanocapsules.

Techniques: Infection, Staining, Control

Journal: bioRxiv

Article Title: Genipin-Crosslinked, Silane-Anchored 3D Tumor–Stroma Microtissues for High-Content On-Chip Drug Testing

doi: 10.1101/2025.07.03.662913

Figure Lengend Snippet: (1A) Schematic of microfluidic chip fabrication. PDMS devices were generated by soft lithography using SU-8 patterned silicon wafers, punched to create inlet/outlet ports, and irreversibly bonded to glass coverslips via oxygen plasma activation. (1B) Hydrogel loading and cell encapsulation workflow. Tumor cells and fibroblasts were co-seeded in a Matrigel/Collagen I/Hyaluronic Acid (Mat/COL/HA) matrix crosslinked with Genipin and injected into the central chamber. Devices were cultured for 6 days to allow microtissue formation prior to 4 days (96⍰h) of drug treatment. (1C) Schematic representation of the microfluidic chip layout showing the spatial distribution of tumor cells and fibroblasts within the central hydrogel chamber and adjacent side channels used for media perfusion and drug delivery. (1D) FTIR-ATR spectra (950–1730⍰cm −1 ) comparing Mat/COL/HA hydrogels with and without Genipin. Key shifts in amide I/II and C–O/C–N stretching regions indicate successful crosslinking and structural rearrangement. (1E) FTIR-ATR spectra (2450–3700⍰cm −1 ) highlighting changes in CH 2 , O–H, and N–H stretching bands. Decreases at 3284 and 2833⍰cm −1 , along with persistent signals, suggest partial retention of functional groups relevant for subsequent surface anchoring. (1F) Schematic representation of the dual-surface functionalization strategy. Plasma-activated PDMS and glass were silanized using APTES to enable covalent bonding to Genipin-crosslinked hydrogels via residual hydroxyl and amine groups.

Article Snippet: Normal human foreskin fibroblasts BJ (ATCC CRL-2522), hTERT-immortalized cancer-associated fibroblasts (PF179T), and LNCaP prostate cancer cells were obtained from the biobank of the Institute of Biomedicine, Cancer Research Unit and FICAN West Cancer Centre Laboratory, University of Turku and Turku University Hospital.

Techniques: Generated, Clinical Proteomics, Activation Assay, Encapsulation, Injection, Cell Culture, Functional Assay

Journal: bioRxiv

Article Title: Genipin-Crosslinked, Silane-Anchored 3D Tumor–Stroma Microtissues for High-Content On-Chip Drug Testing

doi: 10.1101/2025.07.03.662913

Figure Lengend Snippet: (2A) Quantification of hydrogel area retention over 6 days for UT-SCC-19A and UT-SCC-44 cell lines cultured in mono- or co-culture with BJ fibroblasts. Four conditions were assessed: untreated, APTES-only, Genipin-only (500rzµM), and APTES + Genipin. Data represent mean ± SD of three biological replicates. Statistical analysis was performed using two-way ANOVA with Dunnett’s post hoc test versus untreated control. (2B) Brightfield images of UT-SCC-19A/BJ co-cultures in the central chamber at day 6, comparing Genipin-crosslinked and non-crosslinked hydrogels. Scale bar: 200⍰µm. (2C) Immunofluorescence staining of UT-SCC-19A/BJ (top) and UT-SCC-44/BJ co-cultures (bottom) after 10 days, showing Vimentin (mesenchymal marker), pan-Cytokeratin (epithelial marker), F-actin (Phalloidin), and nuclei (Hoechst 33342) stainings. Z-stack projections acquired using Zeiss LSM980 with 10× objective. Scale bar: 100⍰µm.

Article Snippet: Normal human foreskin fibroblasts BJ (ATCC CRL-2522), hTERT-immortalized cancer-associated fibroblasts (PF179T), and LNCaP prostate cancer cells were obtained from the biobank of the Institute of Biomedicine, Cancer Research Unit and FICAN West Cancer Centre Laboratory, University of Turku and Turku University Hospital.

Techniques: Cell Culture, Co-Culture Assay, Control, Immunofluorescence, Staining, Marker

Journal: bioRxiv

Article Title: Genipin-Crosslinked, Silane-Anchored 3D Tumor–Stroma Microtissues for High-Content On-Chip Drug Testing

doi: 10.1101/2025.07.03.662913

Figure Lengend Snippet: (5A) Cisplatin dose– response in UT-SCC-19A and UT-SCC-44 tumor–fibroblast co-cultures embedded in native (non-crosslinked) versus Genipin-crosslinked hydrogels. (5B) Comparison of drug sensitivity in native hydrogels between cells adhered to the chip surface and cells retained within the hydrogel matrix. (5C-E) Dose–response curves for UT-SCC-19A (C) and UT-SCC-44 (E) in monoculture, co-culture with BJ fibroblasts, and co-culture with patient-derived CAFs. Each dot represents a biological replicate (n = 3), with viability expressed as % of untreated control and plotted against log-transformed cisplatin concentrations. (5F) Summary of IC 50 values calculated from nonlinear regression using the four-parameter Hill equation. Bars indicate the median IC 50 (µM), with error bars representing the interquartile range (25th–75th percentile). Individual biological replicate values (n = 3) are shown as black dots; mean IC 50 values are annotated above each bar. (5G) Representative immunofluorescence images of UT-SCC-44 in 3D monoculture and BJ co-culture following 4 days of Cisplatin treatment. Samples were stained for Vimentin (mesenchymal marker), Pan-cytokeratin (epithelial marker), Phalloidin (F-actin), and Hoechst 33342 (nuclei). Z-projected images acquired using a 10× objective on super-resolution microscopy. Scale bar = 100⍰µm. Statistical analysis: two-way ANOVA with Tukey’s post hoc test unless otherwise stated. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Normal human foreskin fibroblasts BJ (ATCC CRL-2522), hTERT-immortalized cancer-associated fibroblasts (PF179T), and LNCaP prostate cancer cells were obtained from the biobank of the Institute of Biomedicine, Cancer Research Unit and FICAN West Cancer Centre Laboratory, University of Turku and Turku University Hospital.

Techniques: Comparison, Co-Culture Assay, Derivative Assay, Control, Transformation Assay, Immunofluorescence, Staining, Marker, Super-Resolution Microscopy